• Product Nameβ-Catenin Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHu Ms Rt
• SpecificityThe β-Catenin Mouse Monoclonal antibody detects endogenous β-Catenin proteins.
• Target Nameβ-Catenin
• SDS-PAGE MW 92kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  Immunohistochemistry: 1:200
  

The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.

• Western blot analysis of 1) Hela, 2) 293T, 3) MCF7, 4)Mouse Brain tissue ,3) Rat LiverTissue, using #38020 diluted at 1:2,000
• IHC staining of human breast cancer tissue with β-Catenin mouse mAb(13C6) diluted at 1:200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting