• Product NameABCB5 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityABCB5 Mouse Monoclonal antibody detects endogenous ABCB5 proteins.
• Target NameABCB5
• Alternative Names    ABCB5; ABCB5 P-gp; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5
• Accession No. Swiss-Prot#:Q2M3G0
• UniprotQ2M3G0
• Gene ID340273;
• SDS-PAGE MW 138kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:2000
  IHC dilution:1:50-300
  IF dilution:1:200

ATP-binding cassette sub-family B member 5 also known as P-glycoprotein ABCB5 is a plasma membrane-spanning protein that in humans is encoded by the ABCB5 gene. ABCB5 is an ABC transporter and P-glycoprotein family member principally expressed in physiological skin and human malignant melanoma.

• Western blot analysis of 1) Hela, 2) 293T, using #38031 diluted at 1:2,000.
• Immunofluorescence analysis of Human-liver-cancer tissue. 1,ABCB5 Monoclonal Antibody(11A2)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,ABCB5 Monoclonal Antibody(11A2) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting