• Product NameCarbonic Anhydrase IX Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityCA IX Mouse Monoclonal antibody detects endogenous CA IX protein.s
• Target NameCarbonic Anhydrase IX
• Alternative Names    CA-IX; CA9; CAH9; CAIX; Car9; Carbonate dehydratase IX; Carbonic anhydrase 9
• Accession No. Swiss-Prot#:Q16790
• UniprotQ16790
• Gene ID768;
• SDS-PAGE MW 49kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:3000~1:5000
  IP dilution: 1:200
  IHC dilution:1:50-300
  IF dilution:1:200

The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors

• Western blot analysis of 1) Hela, 2) 293T, using #38033 diluted at 1:5,000.
• 1¡¢Input: Hela Cell Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #38033 1:2,000
  Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000
• Immunofluorescence analysis of human-liver-cancer tissue. 1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting