• Product NameOct1 Mouse Monoclonal Antibody (27-7G1)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.27-7G1
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu
• SpecificityAntibody detects endogenous Oct1 protein.
• Target Name1-Oct
• Alternative Names    NF-A1; Oct-1; OCT1; Octamer-binding protein 1; Octamer-binding transcription factor 1
• Accession No. Swiss-Prot#:P14859
• UniprotP14859
• Gene ID5451;
• SDS-PAGE MW 90kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:500~1:2000

  
  IHC dilution:1:50-300
  IF dilution:1:200

POU domain, class 2, transcription factor 1 is a protein that in humans is encoded by the POU2F1 gene.Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins.

• Western blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, using #38046
• Immunofluorescence analysis of Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody(7G1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting