• Product NamePeroxiredoxin 1 Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHu Rt Ms
• SpecificityThe Peroxiredoxin 1 Antibody detects endogenous Peroxiredoxin 1 protein.
• Target NamePeroxiredoxin 1
• Alternative Names    MSP23; Natural killer cell-enhancing factor A; natural killer-enhancing factor A; NKEF-A; NKEFA; PAG; PAGA; PAGB
• Accession No. Swiss-Prot#:Q06830
• UniprotQ06830
• Gene ID5052;
• SDS-PAGE MW 21kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB dilution: 1:1000~1:3000
  IF dilution: 1:100-200
  IHC dilution:1:50-300

Peroxiredoxin-1 is a protein which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene.

• Western blot analysis of 1) MCF7, 2) Rat Kidney Tissue, 3) Mouse Brain Tissue, using #38055 diluted at 1:2000.
• IF analysis of Hela with #38055(Left) and DAPI (Right) diluted at 1:100.
• Immunofluorescence analysis of Human-appendix tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunofluorescence analysis of Rat-brain tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Peroxiredoxin 1 Monoclonal Antibody(8E7) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting