• Product NameCarcinoembryonic Antigen Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.1.00E+02
• PurificationAffinity purification using immunogen.
• ApplicationsIHC,IF
• Species ReactivityHu
• SpecificityThe CEA Mouse Monoclonal Antibody detects endogenous CEA proteins.
• Target NameCarcinoembryonic Antigen Antibody
• Alternative Names    CEA;Pregnancy-specific glycoprotein 2; PS-beta-E; PS-beta-G-2; PSBG-2; PSBG2; PSG1; PSG2; PSGGB
• Accession No. Swiss-Prot#:P11465
• UniprotP11465
• Gene ID5670;
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• IHC dilution: 1:200
  
  IF dilution:1:50-200

CEA is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

• IHC staining of human colon cancer tissue with CEA mouse mAb(10E1) diluted at 1:200.
• Immunofluorescence analysis of Human-lung-cancer tissue. 1,Carcinoembryonic Antigen Monoclonal Antibody(10E1)(red) was diluted at 1:200(4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
• Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Carcinoembryonic Antigen Monoclonal Antibody(10E1) was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting