• Product NameHistone H3(Di-methyl-K27) Mouse Monoclonal Antibody (3B12)
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.3B12
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityAntibody detects endogenous Histone H3 (Di-methyl-K27)protein.
• Immunogen DescriptionA synthetic Di-methylated peptide corresponding to residues surrounding K27 of human histone H3.
• Target NameHistone H3(Di-methyl-K27)
• ModificationMethyl
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:3000

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of Hela cells, using #HW046 diluted at 1) 1:1,000, 2) 1:3,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting