• Product NameHistone H3(Tri-methyl-K9) Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB IP
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H3(Tri-methyl-K9) antibody can detects endogenous Histone H3(Tri-methyl-K9) protein.
• Immunogen DescriptionA synthetic Tri-methylated peptide corresponding to residues surrounding K9 of human histone H3.
• Target NameHistone H3(Tri-methyl-K9)
• ModificationMethyl
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:3000
  Immunoprecipitation: 1:200

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of Hela cells, using #HW051 diluted at 1) 1:2,000 2) 1:5,000
• Western blot analysis of 1) Rat Testis Tissue, 2) Raw264.7, using #HW051 diluted at 1:2000.
• 1¡¢Input: Hela Cell Lysate
  2¡¢IP product: IP dilute 1:200
  Western blot analysis: primary antibody : #HW051 1:1,000
  Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting