• Product NameHistone H3(Di-Methyl-Lys4) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H3(Di-methyl-Lys4) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Di-methyl-Lys4) protein.
• Immunogen Typepeptide
• Immunogen DescriptionA synthetic Di-methylated peptide corresponding to residues surrounding Lys4 of human histone H3.
• Target NameHistone H3(Di-Methyl-Lys4)
• ModificationMethyl
• Alternative Names    H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Purified Rabbit IgG, 1mg/ml in PBS pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of 1) Hela, 2)3T3 , 3) Raw264.7, using #HW068 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting