• Product NameHistone H4(Acetyl-Lys12) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H4(Acetyl Lys12) Rabbit Polyclonal Antibody detects endogenous Histone H3 (Acetyl Lys12) protein.
• Immunogen Typepeptide
• Immunogen DescriptionA synthetic acetylated peptide corresponding to residues surrounding Lys12 of human histone H4.
• Target NameHistone H4(Acetyl-Lys12)
• ModificationAcetyl
• Alternative Names    Histone 1 H4 antibody; HIST1H4K antibody; MGC24116 antibody; dJ160A22.1 antibody
• Accession No. Swiss-Prot#:P62805
• UniprotP62805
• Gene ID121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370;
• SDS-PAGE MW 14kd
• Concentration 1.0mg/ml
• Formulation Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

• Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW091 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting