• Product NameHistone H2A.X(Acetyl-Lys5) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H2A.X(Acetyl Lys5) Rabbit Polyclonal Antibody detects endogenous Histone H2A.X(Acetyl Lys5) protein.
• Immunogen Typepeptide
• Immunogen DescriptionA synthetic acetylated peptide corresponding to residues surrounding Lys5 of human histone H2A.X.
• Target NameHistone H2A.X(Acetyl-Lys5)
• ModificationAcetyl
• Alternative Names    H2A histone family member X antidoby; H2AFX antibody; H2AX Histoneh antibody; Histone H2AX antibody
• Accession No. Swiss-Prot#:P16104
• UniprotP16104
• Gene ID3014;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

• Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using #HW093 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting