• Product NameAvi Tag Mouse Monoclonal Antibody(6E3)
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• SpecificityThe Avi-Tag Mouse Monoclonal Antibody detects Avi-Tag recombinant protein.
• Target NameAvi Tag
• Alternative Names    Avi Tag antibody
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 51% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:5000~1:10000
  

The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.

• Western blot analysis of Avi-Recombinant protein, using #T517 diluted at 1) 1:5,000 2) 1:10,000

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting