• Product NameFlag-Tag Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB,IF IP
• Species ReactivityHu Ms Rt
• SpecificityThe Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
• Target NameFlag-Tag
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:5000~1:10000
  Immunofluorescence: 1:2000
  Immunoprecipitation: 1:5000

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

• 1ug Flag fusion protein+ Primary antibody #T519 dilution at 1:10,000.
• IF analysis of 293 cells transfected with a Flag-tag protein, using anti-Flag-Tag Mouse mAb #T519 at a 1:2000 dilution (blue DAPI, red anti-Flag).
• IP antibody use£º5ug Flag Mouse IgG1 per ml Lysate£¬WB 1:5000
  1¡¢untransfected 293 cell lysate
  2¡¢transfected 293 cell lysate with Flag-tag fusion protein
  3¡¢IP (transfected 293+ normal Mouse IgG+Protein G agarose)
  4¡¢IP (transfected 293+anti- Flag mAb+ Protein G agarose)
  5¡¢IP (transfected 293+Protein G)
  6¡¢Recombinant protein (E.coli).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting