• Product Nameβ-actin Mouse Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• PurificationAffinity purification using immunogen.
• ApplicationsWB
• Species ReactivityZebrafish
• SpecificityAntibody detects Zebrafish endogenous β-actin protein.
• Target Nameβ-actin
• Alternative Names    ACTB; BRWS1; PS1TP5BP1
• Accession No. Swiss-Prot#:P60709
• UniprotP60709
• Gene ID60;
• SDS-PAGE MW 45kd
• Concentration 1.0mg/ml
• Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:2000~1:5000
  

β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

• Western blot analysis of Zebrafish skeletal muscle, using #38063 diluted at 1:3,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting