• Product NameHistone H3(Phospho-Ser10) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H3(Phospho Ser10) Rabbit Polyclonal Antibody detects endogenous Histone H3(Phospho Ser10) protein.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 10(R-K-S(p)-T-G) derived from Human Histone H3.
• Target NameHistone H3(Phospho-Ser10)
• ModificationPhospho
• Alternative Names    H3 histone antibody; HIST1H3A antibody; Histone cluster 1; H3a antibody
• Accession No. Swiss-Prot#:P68431
• UniprotP68431
• Gene ID8350;8351;8352;8353;8354;8355;8356;8357;8358;8968;
• SDS-PAGE MW 15-17kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:1000
  

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

• Western blot analysis of extracts from Hela cells, untreated (-) or treated with Nocodazole (1μg/ml, 24 hr; +), using #12084 diluted at 1:1,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting