• Product NameHistone H2A.X(Phospho-Ser139) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H2A.X(Phospho Ser139) Rabbit Polyclonal Antibody detects endogenous Histone H2A.X(PhosphoSer139) protein.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 139(Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
• Target NameHistone H2A.X(Phospho-Ser139)
• ModificationPhospho
• Alternative Names    H2A histone family member X antidoby; H2AFX antibody; H2AX Histoneh antibody; Histone H2AX antibody
• Accession No. Swiss-Prot#:P16104
• UniprotP16104
• Gene ID3014;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation Rabbit Antiserum, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000
  

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

• Western blot analysis of extracts from Hela cells, using #12100 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting