• Product NameHistone H2A.X(Phospho-Tyr142) Rabbit Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Rt Ms
• SpecificityThe Histone H2A.X(Phospho Tyr142) Rabbit Polyclonal Antibody detects endogenous Histone H2A.X(Phospho Tyr142) protein.
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 142(A-S-Q-E-Y(p)) derived from Human Histone H2A.X.
• Target NameHistone H2A.X(Phospho-Tyr142)
• ModificationPhospho
• Alternative Names    H2A histone family member X antidoby; H2AFX antibody; H2AX Histoneh antibody; Histone H2AX antibody
• Accession No. Swiss-Prot#:P16104
• UniprotP16104
• Gene ID3014;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
• Storage Store at -20˚C

• Western blotting: 1:1000~1:2000

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

• Western blot analysis of extracts from Hela cells, using #12101 diluted at 1:2,000.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting