• Product NameCDC25B (Phospho-Ser323) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.
• Target NameCDC25B
• ModificationPhospho
• Alternative Names    CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2
• Accession No. Swiss-Prot#:P30305;
  NCBI Gene#:994
• UniprotP30305
• Gene ID994;
• SDS-PAGE MW 62kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

• Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using CDC25B (Phospho-Ser323) antibody #12104. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting