• Product NameKir6.2 (Phospho-Thr224) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of Kir6.2 only when phosphorylated at threonine 224.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 224 (K-T-TP-S-P) derived from Human Kir6.2.
• Target NameKir6.2
• ModificationPhospho
• Alternative Names    ATP-sensitive inward rectifier potassium channel 11; IKATP; IRK11; Inward rectifier K channel Kir6.2; KCNJ11; Potassium channel; inwardly rectifying; subfamily J; member 11
• Accession No. Swiss-Prot#:Q14654;
  NCBI Gene#:3767
• UniprotQ14654
• Gene ID3767;
• SDS-PAGE MW 40kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunofluorescence: 1:100~1:500

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium By similarity. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

• Western blot analysis of extracts from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The lane on the right is treated with the synthesized peptide.
• Immunofluorescence analysis of HuvEc cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The picture on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting