• Product NameProteasome α3 (Phospho-Ser250) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Proteasome α3 only when phosphorylated at serine 250.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 250 (D-E-S(p)-D-D) derived from Human Proteasome α3.
• Target NameProteasome α3
• ModificationPhospho
• Alternative Names    EC 3.4.25.1; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; proteasome component C8; Proteasome subunit alpha type 3; PSA3; PSC8
• Accession No. Swiss-Prot#:P25788;
  NCBI Gene#:5684
• UniprotP25788
• Gene ID5684;
• SDS-PAGE MW 32kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

• Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Proteasome α3 (Phospho-Ser250) antibody #12113. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting