• Product NameTau (Phospho-Ser516/199) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Tau only when phosphorylated at serine 516/199.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 516/199 (Y-S-S(p)-P-G) derived from Human Tau.
• Target NameTau
• ModificationPhospho
• Alternative Names    MAPT; Microtubule-associated protein tau; MTBT1; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau
• Accession No. Swiss-Prot#:P10636;
  NCBI Gene#:4137
• UniprotP10636
• Gene ID4137;
• SDS-PAGE MW 74kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

• Western blot analysis of extracts from Mouse brain cells, using Tau (Phospho-Ser516/199) antibody #12123. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting