• Product NameCREB (Phospho-Thr100) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of CREB only when phosphorylated at threonine 100.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 100 (S-G-T(p)-Q-I) derived from Human CREB.
• Target NameCREB
• ModificationPhospho
• Alternative Names    cAMP responsive element binding protein 1; cAMP-response element binding protein; CREB-1; CREB1
• Accession No. Swiss-Prot#:P16220;
  NCBI Gene#:1385
• UniprotP16220
• Gene ID1385;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

• Western blot analysis of extracts from HUVEC cells, treated with etoposide (25uM, 24hours), using CREB (Phospho-Thr100) antibody #12133. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting