• Product NameeNOS (Phospho-Ser615) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of eNOS only when phosphorylated at serine 615.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 615 (F-N-S(p)-I-S) derived from Human eNOS.
• Target NameeNOS
• ModificationPhospho
• Alternative Names    Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; NOS; type III; NOS3; NOSIII; Nitric-oxide synthase; endothelial; cNOS
• Accession No. Swiss-Prot#:P29474;
  NCBI Gene#:4846
• UniprotP29474
• Gene ID4846;
• SDS-PAGE MW 140kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

• Western blot analysis of extracts from K562 cells, treated with EGF (40nM, 30mins), using eNOS (Phospho-Ser615) antibody #12137. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting