• Product NameFANCG (Phospho-Ser383) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of FANCG only when phosphorylated at serine 383.
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 383 (R-F-S(p)-P-P) derived from Human FANCG.
• Target NameFANCG
• ModificationPhospho
• Alternative Names    Fanconi anemia group G protein;Protein FACG;DNA repair protein XRCC9
• Accession No. Swiss-Prot#:O15287;
  NCBI Gene#:2189
• UniprotO15287
• Gene ID2189;
• SDS-PAGE MW 69kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

• Western blot analysis of extracts from K562 cells, using FANCG (Phospho-Ser383) antibody #12138. The lane on the right is treated with the synthesized peptide.
• Western blot analysis of extracts from cos-7 cells (Lane 2), using FANCG (Phospho-Ser383) Antibody #12138. The lane on the left is treated with synthesized peptide

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting