• Product NameGab2 (Phospho-Tyr452) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Gab2 only when phosphorylated at Tyr452.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 452 (D-N-Y(p)-V-P) derived from Human Gab2.
• Target NameGab2
• ModificationPhospho
• Alternative Names    GRB2-associated-binding protein 2 GRB2-associated binder 2 Growth factor receptor bound protein 2-associated protein 2 pp100 GAB2 KIAA0571
• Accession No. Swiss-Prot#:Q9UQC2;
  NCBI Gene#:9846
• UniprotQ9UQC2
• Gene ID9846;
• SDS-PAGE MW 75kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis.

• Western blot analysis of extracts from JK, using Gab2 (Phospho-Tyr452) antibody #12139. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting