• Product NameMDM4 (Phospho-Ser367) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of MDM4 only when phosphorylated at serine 367.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 367 (T-I-S(p)-A-P) derived from Human MDM4.
• Target NameMDM4
• ModificationPhospho
• Alternative Names    double minute 4 protein; Mdm2-like p53-binding protein; MDMX; p53-binding protein Mdm4
• Accession No. Swiss-Prot#:O15151;
  NCBI Gene#:4194
• UniprotO15151
• Gene ID4194;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.

• Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MDM4 (Phospho-Ser367) antibody #12141. The lane on the right is treated with the synthesized peptide.
• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using MDM4 (Phospho-Ser367) antibody #12141. The picture on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting