• Product Namep16-INK4a (Phospho-Ser152) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of p16-INK4a only when phosphorylated at serine 152.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 152 (G-P-S(p)-D-I) derived from Human p16-INK4a.
• Target Namep16-INK4a
• ModificationPhospho
• Alternative Names    CD2A1; CDK4I; CDKN2; CDKN2A; CDN2; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A; MTS1; Multiple tumor suppressor 1; p14ARF; p16(INK4a); p16-INK4; P16INK4A
• Accession No. Swiss-Prot#:P42771;
  NCBI Gene#:1029
• UniprotP42771
• Gene ID1029;
• SDS-PAGE MW 20kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

• Western blot analysis of extracts from HeLa cells, treated with EPO (20U/ml, 15mins), using p16-INK4a (Phospho-Ser152) antibody #12148. The lane on the right is treated with the synthesized peptide.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using p16-INK4a (Phospho-Ser152) antibody #12148. The picture on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting