• Product NameUBF (Phospho-Ser484) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of UBF only when phosphorylated at serine 484.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from Human UBF.
• Target NameUBF
• ModificationPhospho
• Alternative Names    Autoantigen NOR-90; Nucleolar transcription factor 1; TCFUBF; UBF-1; UBF1; UBTF; Upstream binding factor 1
• Accession No. Swiss-Prot#:P17480;
  NCBI Gene#:7343
• UniprotP17480
• Gene ID7343;
• SDS-PAGE MW 85kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

• Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using UBF (Phospho-Ser484) antibody #12154. The lane on the right is treated with the synthesized peptide.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBF (Phospho-Ser484) antibody #12154. The picture on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting