• Product NameVitamin D3 Receptor (Phospho-Ser51) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.
• Immunogen Typepeptide
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 51 (R-R-S(p)-M-K) derived from Human Vitamin D3 Receptor.
• Target NameVitamin D3 Receptor
• ModificationPhospho
• Alternative Names    1;25-dihydroxyvitamin D3 receptor; NR1I1; vitamin D receptor; vitamin D3 receptor
• Accession No. Swiss-Prot#:P11473;
  NCBI Gene#:7421
• UniprotP11473
• Gene ID7421;
• SDS-PAGE MW 38kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  

Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

• Western blot analysis of extracts from COLO cells, treated with Insulin (0.01U/ml, 15mins), using Vitamin D3 Receptor (Phospho-Ser51) antibody #12155. The lane on the right is treated with the synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting