• Product NameMDM2(Phospho-Ser260) antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of MDM2 only when phosphorylated at serine 260.
• Immunogen TypePeptide
• Immunogen DescriptionA phospho specific peptide corresponding to residues surrounding S260 of human MDM2.
• Target NameMDM2
• ModificationPhospho
• Alternative Names    HDMX; hdm2; ACTFS; MGC5370; MGC71221
• Accession No. Swiss-Prot#: Q00987
  NCBI Gene ID: 4193
• UniprotQ00987
• Gene ID4193;
• SDS-PAGE MW 95kD;55KD(Calculated)
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

• Western blot analysis of extracts of 293 cell line, using phospho-MDM2-S260 .

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting