• Product NameIRS1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total IRS1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human IRS1.
• Target NameIRS1
• Alternative Names    IRS1;HIRS-1;
• Accession No. Swiss-Prot#: P35568
  NCBI Gene ID: 3667
• UniprotP35568
• Gene ID3667;
• SDS-PAGE MW 132kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:1000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

• Immunohistochemistry of paraffin-embedded human gastric cancer using IRS1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using IRS1 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of HepG2 cells, using IRS1 at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting