• Product NamePIK3CA antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PIK3CA protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PIK3CA.
• Target NamePIK3CA
• Alternative Names    PIK3CA;MGC142161;MGC142163;PI3K;p110-alpha;
• Accession No. Swiss-Prot#: P42336
  NCBI Gene ID: 5290
• UniprotP42336
• Gene ID5290;
• Calculated MW 110kDa
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.

• Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CA at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of HeLa cells using PIK3CA at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of NIH/3T3 cells using PIK3CA at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of HeLa cells, using PIK3CA at 1:500 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting