• Product NameFASN antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total FASN protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human Fatty Acid Synthase (FASN) (NP_004095.4).
• Target NameFASN
• Alternative Names    FAS;OA-519;SDR27X1;FASN
• Accession No. Uniprot:P49327
  GeneID:2194
• UniprotP49327
• Gene ID2194
• SDS-PAGE MW 273KDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

• Immunohistochemistry of paraffin-embedded human breast cancer using Fatty Acid Synthase (FASN) antibody.
• Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody.
• Immunofluorescence analysis of C6 cells using Fatty Acid Synthase (FASN) Rabbit pAb.
• Immunofluorescence analysis of NIH/3T3 cells using Fatty Acid Synthase (FASN) Rabbit pAb.
• Immunofluorescence analysis of U2OS cells using Fatty Acid Synthase (FASN) Rabbit pAb.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting