• Product NameAIF1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total AIF1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human AIF1.
• Target NameAIF1
• Alternative Names    IBA1; IRT1; AIF-1; IRT-1;
• Accession No. Swiss-Prot#: P55008
  NCBI Gene ID: 199
• UniprotP55008
• Gene ID199;
• SDS-PAGE MW 17kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

• Western blot analysis of extracts of rat spleen, using Iba1 at 1:1000 dilution.
• Immunofluorescence analysis of Raw264.7 cells using Iba1 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat bone marrow using Iba1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded rat spleen using Iba1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 at dilution of 1:100 (40x lens).
• Immunofluorescence analysis of Jurkat cells using Iba1 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting