• Product NameITGAM antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total ITGAM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human ITGAM.
• Target NameITGAM
• Alternative Names    CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6;
• Accession No. Swiss-Prot#: P11215
  NCBI Gene ID: 3684
• UniprotP11215
• Gene ID3684;
• SDS-PAGE MW 127kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

ITGAM, also named as CD11B and CR3A, belongs to the integrin alpha chain family. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. ITGAM is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. It is also a receptor for fibrinogen, factor X and ICAM1. ITGAM recognizes P1 and P2 peptides of fibrinogen gamma chain.

• Immunofluorescence analysis of rat bone marrow using CD11B at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of RAW264.7 cells using CD11B at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of THP-1 cells using CD11B at dilution of 1:100. Blue: DAPI for nuclear staining.
• Western blot analysis of extracts of various cell lines, using CD11B at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting