• Product NamePKLR antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• IsotypeIgG
• PurificationAffinity purification
• ApplicationsWB
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total PKLR protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant fusion protein of human PKLR (NP_000289.1).
• Target NamePKLR
• Alternative Names    PKLR;PK1;PKL;PKR;PKRL;RPK
• Accession No. Uniprot:P30613
  GeneID:5313
• UniprotP30613
• Gene ID5313
• SDS-PAGE MW 62kDa
• Concentration 1.0mg/ml
• Formulation PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Storage Store at -20˚C. Avoid freeze / thaw cycles.

• WB 1:500 - 1:2000

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of Raji cells, using PKLR antibody.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting