• Product NameIGFBP4 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total IGFBP4 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human IGFBP4.
• Target NameIGFBP4
• Alternative Names    BP-4; IBP4; IGFBP-4; HT29-IGFBP;
• Accession No. Swiss-Prot#: P22692
  NCBI Gene ID: 3487
• UniprotP22692
• Gene ID3487;
• SDS-PAGE MW 28kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:200

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

• Immunofluorescence analysis of L929 cells using IGFBP4 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat brain using IGFBP4 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human stomach using IGFBP4 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using IGFBP4 at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse brain using IGFBP4 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting