• Product NameMKI67 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total MKI67 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MKI67.
• Target NameMKI67
• Alternative Names    KIA; MIB; MIB-1; PPP1R105;
• Accession No. Swiss-Prot#: P46013
  NCBI Gene ID: 4288
• UniprotP46013
• Gene ID4288;
• SDS-PAGE MW 359kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

• Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 at dilution of 1:200 (40x lens).
• Western blot analysis of extracts of various cell lines, using Ki67 at 1:1000 dilution.
• Immunofluorescence analysis of HeLa cells using Ki67 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting