• Product NameTRAP1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total TRAP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TRAP1.
• Target NameTRAP1
• Alternative Names    HSP75; HSP 75; HSP90L; TRAP-1;
• Accession No. Swiss-Prot#: Q12931
  NCBI Gene ID: 10131
• UniprotQ12931
• Gene ID10131;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.

• Immunohistochemistry of paraffin-embedded mouse heart using TRAP1 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using TRAP1 at 1:3000 dilution.
• Immunofluorescence analysis of NIH-3T3 cells using TRAP1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using TRAP1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat heart using TRAP1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human colon carcinoma using TRAP1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting