• Product NameHNF1A antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total HNF1A protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human HNF1A.
• Target NameHNF1A
• Alternative Names    HNF1; LFB1; TCF1; MODY3; TCF-1; HNF-1A; IDDM20
• Accession No. Swiss-Prot#: P20823
  NCBI Gene ID: 6927
• UniprotP20823
• Gene ID6927;
• Calculated MW 67kd
• SDS-PAGE MW 67kd-81kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100

The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas.

• Western blot analysis of extracts of various cell lines, using HNF1α antibody at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting