• Product NamePELP1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PELP1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human PELP1.
• Target NamePELP1
• Alternative Names    MNAR; P160;
• Accession No. Swiss-Prot#: Q8IZL8
  NCBI Gene ID: 27043
• UniprotQ8IZL8
• Gene ID27043;
• SDS-PAGE MW 119kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:100
  IF 1:50 - 1:100

This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants.

• Western blot analysis of extracts of various cell lines, using PELP1 at 1:1000 dilution.
• Immunofluorescence analysis of L929 cells using PELP1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of U-2 OS cells using PELP1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting