• Product NamePAK1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total PAK1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionA synthetic peptide of human PAK1.
• Target NamePAK1
• Alternative Names    PAKalpha
• Accession No. Swiss-Prot#: Q13153
  NCBI Gene ID: 5058
• UniprotQ13153
• Gene ID5058;
• SDS-PAGE MW 61kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:25 - 1:100

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.?

• Western blot analysis of extracts of mouse kidney, using PAK1 at 1:700 dilution.
• Immunohistochemistry of paraffin-embedded rat spleen using PAK1 at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded human lung cancer using PAK1 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting