• Product NameRIPK3 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total RIPK3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of Human RIPK3.
• Target NameRIPK3
• Alternative Names    RIP3;
• Accession No. Swiss-Prot#: Q9Y572
  NCBI Gene ID: 11035
• UniprotQ9Y572
• Gene ID11035;
• SDS-PAGE MW 56kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.

• Immunohistochemistry of paraffin-embedded mouse pancreas using RIP3 at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using RIP3 at 1:1000 dilution.
• Immunofluorescence analysis of C6 cells using RIP3 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunofluorescence analysis of L929 cells using RIP3 at dilution of 1:100. Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human liver cancer using RIP3 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting