• Product NameDnmt1 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman
• SpecificityThe antibody detects endogenous level of total Dnmt1 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human Dnmt1.
• Target NameDnmt1
• Alternative Names    AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN;
• Accession No. Swiss-Prot#: P26358
  NCBI Gene ID: 1786
• UniprotP26358
• Gene ID1786;
• SDS-PAGE MW 183kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:200
  IP 1:50 - 1:100

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

• Western blot analysis of extracts of various cell lines, using DNMT1 at 1:1000 dilution.
• Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 at 1:1000 dilution.
• Immunofluorescence analysis of 293T cells using DNMT1 at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting