• Product NameTRIM69 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total TRIM69 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human TRIM69.
• Target NameTRIM69
• Alternative Names    Trif; HSD34; RNF36;
• Accession No. Swiss-Prot#: Q86WT6
  NCBI Gene ID: 140691
• UniprotQ86WT6
• Gene ID140691;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:50 - 1:200

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

• Western blot analysis of extracts of mouse liver, using TRIM69 at 1:1000 dilution.
• Immunofluorescence analysis of A549 cells using TRIM69 . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded human kidney using TRIM69 at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting