• Product NameP4HTM antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total P4HTM protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human P4HTM.
• Target NameP4HTM
• Alternative Names    PH4; PH-4; PHD4; EGLN4; HIFPH4; P4H-TM;
• Accession No. Swiss-Prot#: Q9NXG6
  NCBI Gene ID: 54681
• UniprotQ9NXG6
• Gene ID54681;
• SDS-PAGE MW 56kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000

The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified.

• Western blot analysis of extracts of various cell lines, using P4HTM at 1:1000 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting