• Product NameRARS antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC,IF
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total RARS protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human RARS.
• Target NameRARS
• Alternative Names    ArgRS; DALRD1;
• Accession No. Swiss-Prot#: P54136
  NCBI Gene ID: 5917
• UniprotP54136
• Gene ID5917;
• SDS-PAGE MW 75kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IF 1:10 - 1:100

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Arginyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

• Immunohistochemistry of paraffin-embedded human lung cancer using RARS at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse brain using RARS at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using RARS at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
• Immunofluorescence analysis of MCF-7 cells using RARS . Blue: DAPI for nuclear staining.
• Immunohistochemistry of paraffin-embedded rat brain using RARS at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting