• Product NameCIRBP antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CIRBP protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human CIRBP.
• Target NameCIRBP
• Alternative Names    CIRP;
• Accession No. Swiss-Prot#: Q14011
  NCBI Gene ID: 1153
• UniprotQ14011
• Gene ID1153;
• SDS-PAGE MW 18kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  Immunofluorescence: 1:50 - 1:200
  

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.

• Western blot analysis of extracts of various cell lines, using CIRBP antibody.
• Immunofluorescence analysis of HeLa cell using CIRBP antibody. Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting