• Product NameDARS antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IHC
• Species ReactivityHuman,Mouse,Rat
• SpecificityThe antibody detects endogenous level of total DARS protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human DARS.
• Target NameDARS
• Alternative Names    HBSL; aspRS;
• Accession No. Swiss-Prot#: P14868
  NCBI Gene ID: 1615
• UniprotP14868
• Gene ID1615;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IHC 1:50 - 1:200
  IP 1:50 - 1:200

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.

• Immunohistochemistry of paraffin-embedded rat brain using DARS at dilution of 1:100 (40x lens).
• Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS at dilution of 1:100 (40x lens).
• Western blot analysis of extracts of various cell lines, using DARS at 1:1000 dilution.
• Immunohistochemistry of paraffin-embedded mouse brain using DARS at dilution of 1:100 (40x lens).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting