• Product NameMYLK3 antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were purified by affinity purification using immunogen.
• ApplicationsWB,IF
• Species ReactivityHuman,Mouse
• SpecificityThe antibody detects endogenous level of total MYLK3 protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionRecombinant protein of human MYLK3.
• Target NameMYLK3
• Alternative Names    MLCK; MLCK2; caMLCK;
• Accession No. Swiss-Prot#: Q32MK0
  NCBI Gene ID: 91807
• UniprotQ32MK0
• Gene ID91807;
• SDS-PAGE MW 88kd
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500 - 1:2000
  IF 1:50 - 1:100

Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008

• Immunofluorescence analysis of U2OS cells using MYLK3 . Blue: DAPI for nuclear staining.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting